Three Brazilian sisters have entered the Guinness Book of Records as the oldest living sisters in the world, becoming the focus of researchers' interest in a study examining the genetic and environmental factors associated with longevity. The total age of the three sisters is 316 years, with the eldest, Levita, being 109 years old, followed by Zoraidi, who is 104 years old, while the youngest, Zulina, is 103 years old. The sisters became part of the University of São Paulo's DNA Longevo project, which aims to study genes associated with longevity and good health later in life. In this context, Ben Myers, President of LongeviQuest, explained that this exceptional case indicates the possibility of strong genetic factors that contributed to the sisters reaching these advanced ages, in addition to the family support and social cohesion that they maintained over decades. On the other hand, the sisters attribute the secret of their longevity to the simple lifestyle they lived, continuous physical activity, and their reliance on fresh and natural foods during their childhood, before the spread of modern refrigeration methods. The sisters were born and raised in a small rural town, where from a young age they were accustomed to doing agricultural work and helping the family in the fields, making physical activity an essential part of their daily lives. The eldest sister, Levita, played a pivotal role within the family, as she assumed major household responsibilities and took care of her younger siblings, while Zulina was famous for her skill in sewing and handicrafts, which helped her maintain her independence for many years. Rural life and reliance on fishing and swimming in rivers also contributed to enhancing their physical fitness and establishing healthy habits that continued with them throughout their lives. The researchers also noted that the sisters had great psychological flexibility and a high sense of satisfaction with life, which Levita expressed by saying that she lived her life without regret, content with what she and her family had achieved. Scientists believe that the secret of this rare condition may lie in an exceptional combination of good genes, early physical activity, strong social relationships, and psychological stability, which are factors combined that may help maintain physical and mental health in advanced age.